UVR5-UI is an open-source project based on python-audio-separator, providing a user-friendly interface for separating different tracks in audio files. It employs various models to achieve high-quality audio separation. This project is particularly suitable for music creators, audio editors, and anyone who needs to remove or isolate specific sounds from audio. UVR5-UI supports batch audio separation from multiple websites and can be run on Colab and Kaggle, offering great convenience to users.